PZLAST-MAG

1. Submit a new job.

Query File: Only a FASTA-formatted file for **protein** sequences is acceptable. (not for DNA sequences) Either a single FASTA or MultiFASTA-formatted file is accepted as input. Paste the input sequence into the box (B) or click the button (C) to select a file.
Parameters: PZLAST has the following parameters.
"Output top N hits" (D): This controls how many hits will be output per input sequence. You can set it for top hit (output=1) only, but higher values will give more enjoyable results.
E-value cut-off (E): You can set the cutoff value for the E-value, which defaults to 1e-8. Lowering this value allows you to narrow down the search results to sequences with higher similarity, although it may result in fewer hits.; Press the submit button (F) to register the job.
Input file size limit: All sequences must be >= 10 AA (amino acids) and <= 2,000 AA. The number of input sequences must be <= 10,000. The number of total amino acids must be <= 100,000. The number of possible output must be <= 1,000,000.

After registering the job, the screen changes to the job status page.

This page shows whether the job is currently WATING to execute the calculation on ZettaScaler-3.0 or is RUNNING on it.
This page is refreshed every few seconds. If the calculation is completed (or unfortunately ends with an error or no-hit), this page will automatically transition to the results page.

All jobs are managed with a unique job ID issued at the time of registration.
If you leave this page, press the copy button (A) and copy the job ID to the clipboard (or somewhere else). You can return to this page again by click the "Result" in the navigation tab (B) and entering the job ID. You can also go back to past jobs from the "History" page (C). Please note that jobs are deleted two weeks after registration.

Note: This site uses cookies to record your job history. Only job ID and registration time are recorded in cookie. If "History" is not displayed, please enable cookies in your browser.

Click the "REMOVE THIS JOB" button (D) if you want to delete the waiting job.

2. Result pages.

Information page: When the calculation is completed, this page will be displayed first.

The total number of hits (or the sum of hits when multi-FASTA is input) is displayed in (A).

Press button (B) to download all results in CSV format.

Press button (C) to download all the hit reference sequences in Multi-FASTA format.

The input information is displayed in (D). All results are distinguished by the sequence ID displayed in "Queries", so remember these IDs when checking the results page below.

Table results: Search results are displayed in a similar tabular format as tools such as BLAST. By default, hit records are arranged in ascending order of E-value per input sequence.

(A) is the ID of the input sequence. Clicking on the header of this column will sort the table by query names.
(B) is the Genome ID of MAG. Click to jump to the corresponding page of Microbiome Datahub or EMBL-EBI MGnify (depending on which DB the genome data originated from).
(C) For each record, expand the reference protein sequence that the query hit, and the alignment details.
The letters in the alignment mean the following:
'|' ... Match
':' ... BLOSUM62 score > 0
'.' ... BLOSUM62 score = 0
'*' ... BLOSUM62 score < 0

PZLAST-MAG Help